anti insulin receptor substrate 1  (Cell Signaling Technology Inc)

Journal: Nutrition Research and Practice

Article Title: Vaccinium oldhamii fruits improve insulin resistance by inhibiting inflammation in macrophages and adipocytes

doi: 10.4162/nrp.2025.19.6.839

Figure Lengend Snippet: VOW, Vaccinium oldhamii water extract; LPS, lipopolysaccharide; p-ERK, phosphorylated extracellular signal-regulated kinase; ERK, extracellular signal-regulated kinase; p-JNK, phosphorylated Jun N-terminal kinase; JNK, Jun N-terminal kinase; p-p38, phosphorylated p38; p-IKKα/β, phosphorylated IKKα/β; NF-κB, nuclear factor kappa-light-chain-enhancer of activated B cells; iNOS, inducible nitric oxide synthase; COX-2, cyclooxygenase-2; NO, nitric oxide.

Article Snippet: Primary antibodies (phosphorylated extracellular signal-regulated kinase [p-ERK, #4370; Cell Signaling Technology; CST, Beverly, MA, USA], extracellular signal-regulated kinase [ERK, #4695; CST], phosphorylated JNK [p-JNK, #9255; CST], JNK [#9252; CST], phosphorylated p38 [p-p38, #4511; CST], p38 [#8690; CST], phosphorylated IKKα/β [p-IKKα/β, #2697; CST], IKKα [#61294; CST], NF-κB [#8242; CST], inducible nitric oxide synthase [iNOS, #13120; CST], cyclooxygenase-2 [COX-2, #4842; CST], glucose transporter type 4 [GLUT4, #2213; CST], phosphorylated AK strain transforming [p-AKT, #9271; CST], Ak strain transforming [AKT, #9272; CST], phosphorylated phosphatidylinositol 3-kinase [p-PI3K, #4228; CST], phosphatidylinositol 3-kinase [PI3K, #4292; CST], phosphorylated insulin receptor substrate [p-IRS (Ser 307), #2381; CST], p-IRS (Tyr 632) [sc-17196; Santa Cruz, Dallas, TX, USA], and insulin receptor substrate [#2382; CST]) overnight with horseradish peroxidase-conjugated secondary antibodies (goat anti-rabbit or goat anti-mouse [1:10,000]).

Techniques:

Journal: Nutrition Research and Practice

Article Title: Vaccinium oldhamii fruits improve insulin resistance by inhibiting inflammation in macrophages and adipocytes

doi: 10.4162/nrp.2025.19.6.839

Figure Lengend Snippet: VOW, Vaccinium oldhamii water extract; GLUT4, glucose transporter type 4; p-AKT, phosphorylated AK strain transforming; AKT, AK strain transforming; p-PI3K, phosphorylated phosphatidylinositol 3-kinase; PI3K, phosphatidylinositol 3-kinase; p-IRS, phosphorylated insulin receptor substrate; IRS, insulin receptor substrate.

Article Snippet: Primary antibodies (phosphorylated extracellular signal-regulated kinase [p-ERK, #4370; Cell Signaling Technology; CST, Beverly, MA, USA], extracellular signal-regulated kinase [ERK, #4695; CST], phosphorylated JNK [p-JNK, #9255; CST], JNK [#9252; CST], phosphorylated p38 [p-p38, #4511; CST], p38 [#8690; CST], phosphorylated IKKα/β [p-IKKα/β, #2697; CST], IKKα [#61294; CST], NF-κB [#8242; CST], inducible nitric oxide synthase [iNOS, #13120; CST], cyclooxygenase-2 [COX-2, #4842; CST], glucose transporter type 4 [GLUT4, #2213; CST], phosphorylated AK strain transforming [p-AKT, #9271; CST], Ak strain transforming [AKT, #9272; CST], phosphorylated phosphatidylinositol 3-kinase [p-PI3K, #4228; CST], phosphatidylinositol 3-kinase [PI3K, #4292; CST], phosphorylated insulin receptor substrate [p-IRS (Ser 307), #2381; CST], p-IRS (Tyr 632) [sc-17196; Santa Cruz, Dallas, TX, USA], and insulin receptor substrate [#2382; CST]) overnight with horseradish peroxidase-conjugated secondary antibodies (goat anti-rabbit or goat anti-mouse [1:10,000]).

Techniques:

Journal: Nutrition Research and Practice

Article Title: Vaccinium oldhamii fruits improve insulin resistance by inhibiting inflammation in macrophages and adipocytes

doi: 10.4162/nrp.2025.19.6.839

Figure Lengend Snippet: MCM, macrophage-conditioned media; VOW, Vaccinium oldhamii water extract; GLUT4, glucose transporter type 4; p-AKT, phosphorylated AK strain transforming; AKT, AK strain transforming; p-PI3K, phosphorylated phosphatidylinositol 3-kinase; PI3K, phosphatidylinositol 3-kinase; p-IRS, phosphorylated insulin receptor substrate; IRS, insulin receptor substrate.

Article Snippet: Primary antibodies (phosphorylated extracellular signal-regulated kinase [p-ERK, #4370; Cell Signaling Technology; CST, Beverly, MA, USA], extracellular signal-regulated kinase [ERK, #4695; CST], phosphorylated JNK [p-JNK, #9255; CST], JNK [#9252; CST], phosphorylated p38 [p-p38, #4511; CST], p38 [#8690; CST], phosphorylated IKKα/β [p-IKKα/β, #2697; CST], IKKα [#61294; CST], NF-κB [#8242; CST], inducible nitric oxide synthase [iNOS, #13120; CST], cyclooxygenase-2 [COX-2, #4842; CST], glucose transporter type 4 [GLUT4, #2213; CST], phosphorylated AK strain transforming [p-AKT, #9271; CST], Ak strain transforming [AKT, #9272; CST], phosphorylated phosphatidylinositol 3-kinase [p-PI3K, #4228; CST], phosphatidylinositol 3-kinase [PI3K, #4292; CST], phosphorylated insulin receptor substrate [p-IRS (Ser 307), #2381; CST], p-IRS (Tyr 632) [sc-17196; Santa Cruz, Dallas, TX, USA], and insulin receptor substrate [#2382; CST]) overnight with horseradish peroxidase-conjugated secondary antibodies (goat anti-rabbit or goat anti-mouse [1:10,000]).

Techniques: